Which microorganism is used for production of L-asparaginase?
Currently, L-Asparaginase is industrially produced mainly from prokaryotic microbes such as Escherichia coli and Erwinia chrysanthemi.
How asparaginase is produced?
L-asparaginase is produced from a variety of microbial sources including fungi, yeast, bacteria and actinomycetes by the process of submerged fermentation8. Currently, L-asparaginases derived from two bacterial sources, Erwinia chrysanthemi and E. coli, are in clinical use for the treatment of ALL9.
Which of the following marine bacteria showed enhanced production of L-asparaginase?
The marine bacterium B. velezensis isolated from the Red Sea is a valuable potential producer of l-asparaginase. Enzyme production by immobilized cells was 61.04% higher than that by free cells under optimized conditions: 72 h, pH 6.5, 37°C, 100 rpm, with glucose and NH4Cl as carbon and nitrogen sources, respectively.
Is asparaginase a protein?
Asparaginase is a bacterial enzyme. The purpose of this enzyme is to reduce the presence of asparagine. Asparagine is a particular amino acid with one goal; to aid the growth of some tumor cells. Therefore, asparaginase is commonly used as a cancer treatment to slow down and shrink tumors.
What type of enzyme is L asparaginase?
l-asparaginase (EC 3.5. 1.1) is an enzyme that catalysis mainly the asparagine hydrolysis in l-aspartic acid and ammonium. This enzyme is presented in different organisms, such as microorganisms, vegetal, and some animals, including certain rodent’s serum, but not unveiled in humans.
What is the mechanism of action of asparaginase?
Asparaginase works by breaking down the amino acid known as asparagine without which the cancer cells cannot make protein.
What is L asparagine used for?
Asparaginase is used to treat acute lymphocytic leukemia (ALL). It works by starving tumor cells of needed nutrients and slowing tumor cell growth.
What is the use of asparaginase?
Asparaginase is a chemotherapy drug. You might have it as a treatment for acute lymphoblastic leukaemia (ALL). There are 3 types of asparaginase: asparaginase made from a type of bacteria called Escherichia coli (also known by its brand Spectrila)
What type of enzyme is asparaginase?
Asparaginase is a bacterial enzyme that reduces the availability of asparagine, an amino acid that is necessary for the growth of some tumor cells.
Where is L-asparaginase found?
Asparaginase is isolated from Escherichia coli (EC-2 form) and Erwinia chrysanthemi. It contains four identical subunits. Lyophilized asparaginase is a white crystalline powder or plug that is stored under refrigeration. Avoid contact with skin, mucous membranes or eyes, and avoid inhaling the dust or vapor.
Why is asparaginase used in food?
The purpose of using asparaginase in foods is to reduce the levels of acrylamide that form in certain carbohydrate-rich foods during cooking.
Who discovered L-asparaginase?
Following the seminal discovery by Kidd in 1953,[5] asparaginase has been a mainstay of pediatric chemotherapy protocols to treat patients with ALL.