Which organisms can be studied in BSL-1?

Which organisms can be studied in BSL-1?

The agents require Biosafety Level 1 containment. Examples of BSL-1 organisms are: Agrobacterium radiobacter, Aspergillus niger, Bacillus thuringiensis, Escherichia coli strain K12, Lactobacillus acidophilus, Micrococcus leuteus, Neurospora crassa, Pseudomonas fluorescens, Serratia marcescens.

What is considered a BSL-1?

BSL-1. If you work in a lab that is designated a BSL-1, the microbes there are not known to consistently cause disease in healthy adults and present minimal potential hazard to laboratorians and the environment. An example of a microbe that is typically worked with at a BSL-1 is a nonpathogenic strain of E. coli.

What is the lowest BSL that requires?

BSL–1. As the lowest of the four, biosafety level 1 applies to laboratory settings in which personnel work with low-risk microbes that pose little to no threat of infection in healthy adults.

Which is an example of a secondary barrier for BSL-1 laboratories?

Examples of secondary barriers include work areas that are separate from public areas, decontamination facilities, handwashing facilities, special ventilation systems, and airlocks.

What BSL is Ebola?

BIOSAFETY LEVEL 4

BIOSAFETY LEVEL 4 (BSL-4) LABS
Work with the world’s most deadly agents, including viruses that cause smallpox and viral hemorrhagic fevers, such as Ebola, is done at biosafety level 4 (BSL-4).

What BSL level is smallpox?

Biosafety Level 4
Biosafety Level 4 criteria are used when working with smallpox. Smallpox is very contagious and easily spread from person to person. Always use appropriate personal protective equipment including masks, laboratory coats, gloves, and eye or face shields when collecting and handling specimens.

Is E coli a BSL-2 organism?

Organisms. The organisms that require BSL-2 laboratories include the pathogenic strains of E. coli, Staphylococcus, Salmonella, Plasmodium falciparum, Toxoplasma, and Herpes Simples Viruses.

What biosafety level is E coli?

BIOSAFETY LEVEL 2
(CDC) 21-1112) from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention and National Institutes of Health.

Are gloves a primary barrier?

(Secondary Barriers)
PPE: lab coats; gloves; eye/face protection as needed. Primary barriers: BSCs or other physical containment devices used for all open manipulations of agents.

Is PPE primary containment?

This chapter addresses some of the more common primary containment devices and personal protective equipment (PPE) and a variety of equipment-associated hazards. Other chapters cover respiratory protection, work practices, and BSCs that are more specific examples of primary containment.

What Biosafety Level is E coli?

What BSL level is chlamydia?

2/3
How to use this tool

Bacterial Agent	BSL
Chlamydia pneumoniae	2/3
Chlamydia trachomatis	3
Clostridium botulinum*	2/3
Clostridium tetani	2

What are the 4 main PPE?

Components of Personal Protective Equipment (PPE)

• Gloves. Gloves help protect you when directly handling potentially infectious materials or contaminated surfaces.
• Gowns.
• Shoe and Head Covers.
• Masks and Respirators.
• Other Face and Eye Protection.

What stops primary Xray beams?

– Primary radiation barriers protect from primary x-ray beam exposure. These include the doors, lead lined walls, and floors. Secondary radiation barriers are those that protect against scattered radiation (secondary).

What are the 3 PPE for lab?

PPE can be as basic as eye protection (safety glasses or goggles), gloves, and a lab coat or as complex as a Biosafety Level 4 “positive pressure suit” that completely isolates the employee from the laboratory environment. The use of specific PPE required is determined through a risk assessment.

What PPE should be removed first?

Gloves
The order for removing PPE is Gloves, Apron or Gown, Eye Protection, Surgical Mask. Perform hand hygiene immediately on removal. All PPE should be removed before leaving the area and disposed of as healthcare waste.

What are 5 PPE’s?

PPE includes gloves, gowns, laboratory coats, face shields or masks, eye protection, resuscitation masks, and other protective gear such as hats and booties.

What does ALARA stand for?

as low as reasonably achievable
ALARA stands for “as low as reasonably achievable”. ALARA means avoiding exposure to radiation that does not have a direct benefit to you, even if the dose is small. To do this, you can use three basic protective measures in radiation safety: time, distance, and shielding.

What is the leakage radiation limit?

Leakage radiation is limited by regulation to 100 milliroentgens per hour (mR h-1) at 1 m. * This is equal to 0.875 milligray per hour (mGy h-1). The maximum leakage is created when the x-ray tube is operated at its highest tube voltage and at a tube current that it can continuously operate.

What are the 10 most important lab safety rules?

The 10 Most Important Lab Safety Rules

• of 10. The Most Important Lab Safety Rule.
• of 10. Know the Location of Safety Equipment.
• of 10. Dress for the Lab.
• of 10. Don’t Eat or Drink in the Laboratory.
• of 10. Don’t Taste or Sniff Chemicals.
• of 10. Don’t Play Mad Scientist in the Laboratory.
• of 10. Dispose of Lab Waste Properly.
• of 10.

What is bsl3?

Biosafety Level 3 (BSL-3)​
BSL-3 laboratories are used to study infectious agents or toxins that may be transmitted through the air and cause potentially lethal infections. Researchers perform all experiments in a biosafety cabinet. BSL-3 laboratories are designed to be easily decontaminated.

How do you remember donning and doffing?

Do your employees find it difficult to remember the order in which they should put on and take off PPE?
…
Use the following mnemonic tip:

1. Gown.
2. Mask.
3. Goggles.
4. Gloves (when raised above the head)

What PPE is used for diarrhea?

– Managing residents with bladder and bowel incontinence, including diarrhea (addressing use of gown and gloves). – Responding to an emergency in a resident’s room where uncontrolled blood and other body fluids exposure may occur (addressing use of gloves, fluid-resistant gown, facemask, goggles, or a face shield).

What is the 10 day rule?

The guiding principle is the 10-day rule stating that the abdominal area (lumbar spine, pelvis, coccyx, and hips) should not be irradiated after the 1st 10 days of one’s menstrual cycle.

What is the 28 day rule in radiography?

If pregnancy cannot be excluded the 28 day rule applies
The vast majority of routine diagnostic examinations fall into the category of a low dose procedure. If pregnancy cannot be excluded but the menstrual period is not overdue according to the 28 day rule proceed with the examination.