What is the weight of BSA?
Physical properties of BSA: Number of amino acid residues: 583. Molecular weight: 66,463 Da (= 66.5 kDa)
How many kDa is BSA?
BSA is a 585 amino acids, 66 kDa globular protein comprised of at least 67% α-helical secondary structure in physiological conditions .
What is the size of BSA protein?
Being the most abundant serum protein in bovine blood, which is a byproduct of the cattle industry, BSA is a relatively cheap and easily accessible compound. According to the literature, BSA has a nominal size of 7.1 nm and a molecular mass of 66.5 kDa.
What is the molecular weight of bovine albumin?
It has a molecular mass of 66.5 kDa.
What is standard BSA?
BSA is the universally accepted reference protein for total protein quantitation. The albumin standard is precisely formulated at 2 mg/mL in an ultrapure 0.9% sodium chloride (saline) solution.
What is BSA formula?
Formulas to estimate body surface area (BSA) Boyd* BSA (m2) = 0.0003207 x Ht (cm)0.3 x Wt (g)(0.7285 – ( 0.0188 x LOG(Wt (g)))) DuBois and DuBois* BSA (m2) = Wt (kg)0.425 x Ht (cm)0.725 x 0.007184.
How do you convert KDa to molecular weight?
To understand how to do a protein molarity calculation, let’s begin with important conversions. 1 Dalton (Da) = 1 g/mol, this means that 1 KDa = 1000 g/mol = 1 kg/mol.
Why is BSA called Fraction V?
BSA is often referred to as “Fraction V”, based on the original fractionation of human plasma developed by Edwin Cohn. Primarily developed for human albumin production, the process was later adapted to bovine serum albumin.
Why is BSA used as standard for protein content determination?
Generally, bovine serum albumin (BSA) works well for a protein standard because it is widely available in high purity and relatively inexpensive.
Why do we do BSA standard curve for protein estimation?
The protein standard is measured, and its absorbance and concentration are plotted on a graph to create the standard curve. This curve is then used to determine the concentration of the unknown protein.
How is BSA measured?
It calculates BSA by taking the square root of height (cm) multiplied by weight (kg) divided by 3600. Many online calculators offer the conversion from centimeters to inches and from kilograms to pounds, which makes calculating one’s own BSA fairly easy.
What is the unit for BSA?
Various calculations have been published to arrive at the BSA without direct measurement. In the following formulae, BSA is expressed in m2, weight (or, more properly, mass) W in kg, and height H in cm.
Is kDa molecular weight?
Measure of molecular weight or molecular mass. One molecular hydrogen molecular atom has molecular mass of 1 Da, so 1 Da = 1 g/mol. Proteins and other molecular macromolecule molecular weights are usually measured in molecular kDa or kD (kilodaltons) – 1000 Da.
Is kDa a MW?
Yes, 1 KDa is 1000 g/mol, which is the same as 1 kg/mol. Molecular weight is unit-less.
Why does BSA stabilize proteins?
BSA stabilizes Streptococcus thermophilus beta-galactosidase against thermal inactivation and binds to the active enzyme subunits formed on heating. The mechanism of interaction and stabilization, however, is unknown, and it was investigated using different proteins.
What is the purpose of a BSA standard?
Bovine Serum Albumin (BSA) is the standard reference for total protein quantitation by colorimetric assays. Expedeon BSA standards are designed for serial dilution to generate accurate standard curves and are precisely formulated at 2mg/ml.
What is the function of BSA?
Bovine Serum Albumin (BSA) is used in a variety of laboratory applications including its function as a protein concentration standard, its function as a cell nutrient and its ability to stabilize enzymes during restriction digest.
Why is BSA a blocking agent?
The primary role of BSA is to prevent the non-specific binding by blocking the leftover spaces over solid surface after immobilization of a capture biomolecule.
What is BSA standard curve?
A standard curve was developed using a series of Bovine Serum Albumin (BSA) standards in the 100 μg/ml to 1,500 μg/ml range. The absorbance of each sample was measured at 595 nm and plotted versus [BSA]. The resulting line was fit by the linear least squared method.
What BSA means?
Boy Scouts of America.
Is K the same as kDa?
One thousand daltons. A dalton is the weight of a hydrogen atom. The kilodalton is the standard unit used to represent the weight of large molecules such as proteins. It is normally abbreviated to K or Kd.
What unit is kDa?
Dalton (Da) is an alternate name for the atomic mass unit, and kilodalton (kDa) is 1,000 daltons. Thus a peptide with a mass of 64kDa has a molecular weight of 64,000 grams per mole.
What is the unit KDA?
How do you convert KDA to molecular weight?
1 Dalton (Da) = 1 g/mol, this means that 1 KDa = 1000 g/mol = 1 kg/mol.