What is A549 lung cancer cells?
The A549 cell line is a widely used human lung adenocarcinoma cell line that was derived from a primary lung tumor. A549 cells are highly proliferative and have been used in a wide variety of research applications. The A549 cell line is hypotriploid with a modal chromosome number of 66, which occurs in 24% of cells.
What type of cell line is MDA MB 231?
epithelial
The MDA-MB-231 cell line is an epithelial, human breast cancer cell line that was established from a pleural effusion of a 51-year-old caucasian female with a metastatic mammary adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical research laboratories.
Is A549 a Nsclc?
Today a large number of human non-small cell lung cancer cell lines exist that are being used for both basic research and drug discovery.
What mutations do A549 cells have?
The A549 cell line is in turn wild-type against other common mutations in lung cancer such as, EGFR, PIK3CA, TP53, ALK and PTEN. The SW900 cell line is also wild-type for these mutations with the exception for the inactivated mutation in the tumor suppressor gene TP53 (homozygous, c.
Are A549 cells metastatic?
Among NSCLC cells, A549 formed the least amount of metastatic lesions (0.9 ± 0.35 cells per HPF), while H1299, a metastatic cell line, formed the maximum number of metastatic lesions (3.6 ± 1.03 cells per HPF).
Is A549 resistant to cisplatin?
Conclusions: This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells feature an increased sensitivity to pemetrexed treatment.
What is the difference between MDA-MB-231 and MDA-MB-468?
MDA-MB-468 is a basal BC cell line while MDA-MB-231 is a claudin-low cell line – a point Hamed Helal made above, to his credit – with claudin-3, claudinin-4 and claudinin-7 low, and typically with low Ki67 and E-cadherin, in contrast to MDA-MB-468 absent these and itself with high Ki-67 and as I noted, MDA-MB-231 shows …
What is the difference between MCF-7 and MDA-MB-231?
The MCF-7 cell line has functional estrogen and EGF receptors, is dependent on estrogen and EGF for growth, and is uninvasive, while MDA-MB-231 cells are a model for more aggressive, hormone-independent breast cancer.
What are calu 3 cells?
Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene.
What does a cell line mean?
Cell line is a general term that applies to a defined population of cells that can be maintained in culture for an extended period of time, retaining stability of certain phenotypes and functions. Cell lines are usually clonal, meaning that the entire population originated from a single common ancestor cell.
What is hepg2 cell line?
Hep-G2 is a human liver cancer cell line that was derived from the liver tissue of a 15 year old Caucasian male with a hepatocellular carcinoma.
What is Vero cell line?
Vero cells are derived from the kidney of an African green monkey, and are one of the more commonly used mammalian continuous cell lines in microbiology, and molecular and cell biology research.
What is MDA MB 468 cell line?
MDA-MB-468 is a cell line with epithelial morphology that was isolated from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast. These cells have applications in breast cancer and immuno-oncology research.
Is MCF 7 triple negative?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
What are MCF10A cells?
MCF10A cells are widely used non-malignant breast epithelial cells. They exhibit some features of normal breast epithelium, including lack of anchorage-independent growth and dependence on growth factors and hormones for proliferation and survival [6].
What does calu 3 stand for?
Cultured Human Airway Epithelial Cells
Cultured Human Airway Epithelial Cells (Calu-3): A Model of Human Respiratory Function, Structure, and Inflammatory Responses – PMC. An official website of the United States government.
What are Huh 7 cells?
Huh-7 is an immortal cell line composed of epithelial-like, tumorigenic cells. The majority of Huh-7 cells show a chromosome number between 55 and 63 (mode 60) and are highly heterogeneous. These cells are adherent to the surface of flasks or plates and typically grow as 2D monolayers.
Where do human cell lines come from?
Human cell lines are usually obtained from a patient and therefore represent the diseased organ or tissue from that one patient.
How long can cell lines last?
Cell lines can be finite or continuous. An immortalized or continuous cell line has acquired the ability to proliferate indefinitely, either through genetic mutations or artificial modifications. A finite cell line has been sub-cultured for 20-80 passages after which they senesce.
What is HT 29 cell line?
Description. HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. These cells are sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which are standard treatment options for colorectal cancer.
What is HCT116 cell line?
HCT116 (ATCC® CCL-247™) is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases.
Why are BHK 21 cells used?
The World Organization for Animal Health (OIE) uses these cells for routine diagnosis of rabies. This cell line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII, see ATCC CRL-8544).
Why Vero cell is used?
In addition, Vero cells have been licensed in the United States for production of both live (rotavirus, smallpox) and inactivated (poliovirus) viral vaccines, and throughout the world Vero cells have been used for the production of a number of other viruses, including Rabies virus, Reovirus and Japanese encephalitis …
What is the difference between MDA-MB-231 and MDA MB 468?
What is the difference between MCF 7 and MDA-MB-231?