What is a HIV Ag Ab Combo test?
The Determine HIV–1/2 Ag/Ab Combo is an in vitro, visually read, qualitative immunoassay for the simultaneous detection of Human Immunodefciency Virus Type 1 (HIV-1) p24 antigen (Ag) and antibodies (Ab) to HIV Type 1 and Type 2 (HIV-1 and HIV-2) in human serum, plasma, capillary (fngerstick) whole blood or venipuncture …
What is BioPlex used for?
The Bio-Plex® system utilizes xMAP technology to permit the multiplexing of up to 100 different analytes. Multiplex analysis gives researchers the ability to look at analytes simultaneously providing more information from less sample volume in less time than traditional immunoassay methods.
What is HIV Ag AB screen by Cmia?
The ARCHITECT HIV Ag/Ab Combo assay is a chemiluminescent microparticle immunoassay (CMIA) for the simultaneous qualitative detection of human immunodeficiency virus (HIV) p24 antigen and antibodies to HIV type 1 (HIV-1 group M and group O) and/or type 2 (HIV-2) in human serum and plasma (EDTA and heparin).
What BioPlex 2200?
The BioPlex 2200 automated analyzer (Bio-Rad Laboratories, Hercules, CA) is a recently developed multiplex analyzer that enables the detection of anti-Toxoplasma, -rubella, and -cytomegalovirus antibodies in the same assay.
What is a BioPlex assay?
Bio-Plex Multiplex Immunoassays allow you to simultaneously quantify up to 500 proteins, peptides, and nucleic acid targets in a single 12.5 µl sample. Assays for over 450 biologically relevant targets are available as ready-to-use premixed multiplex panels and singleplex sets or in custom-mixed panels.
How does the BioPlex 2200 work?
BioPlex 2200 immunoassays use magnetic 8 µm beads, infused with varying ratios of fluorescent dyes to create unique bead sets. Beads within each set are coated with a ligand (i.e., antigen, antibody, analyte, etc.) specific to a particular assay.
How does a Luminex assay work?
Using a Luminex instrument, beads are excited by one laser to determine the bead region and corresponding assigned analyte. Another laser determines the magnitude of the PE-derived signal, which is proportional to the amount of analyte bound. Multiple readings are taken at each bead region, ensuring robust detection.
Is Luminex an ELISA?
Key differences between ELISA and LUMINEX assays are the use of different target capture systems and different reporter systems. LUMINEX assays capture targets onto spherical beads in suspension while ELISAs generally rely on flat surfaces in multi-well plates to capture targets.
What is Luminex blood test?
Luminex xMAP (Multi-Analyte Profiling) Clinical Information. Test is used for the detection and identification of antibodies directed to donor-specific HLA class I antigens by single-antigen bead-based flow cytometry.
Is Luminex more sensitive than ELISA?
Conclusions: Different methods used to detect HLA antibodies showed discrepant results. As the Luminex method was more sensitive than ELISA for the detection of HLA antibodies, it can be used as a routine test in the transplantation laboratory.
What does Luminex mean?
A Luminex assay is a type of immunoassay that precisely measures multiple analytes in one sample. The Luminex® xMAP® technology is a bead-based immunoassay that allows for multiplex detection of up to 100 analytes simultaneously.
What does it mean if I have HLA antibodies?
HLA antibodies are typically produced after exposure to transplanted tissue, pregnancy, and blood products. Sensitization delays access to transplantation and preclude utilization of donor organs. Infections and vaccinations have also been reported to result in HLA antibody formation.
How are HLA antibodies identified?
The Antibody Specificity Profile assays for antibodies to HLA Class I antigens using the HLA Single Antigen Class I Luminex Bead Test. This test is used to assess a patient’s sensitization to platelet donor HLA. Antibodies to antigen specificities are identified and reported as positive or negative.
What is Luminex used for?
Luminex Platform
Luminex multiplex assays use xMAP bead-based technology to simultaneously detect and quantitate multiple secreted proteins including cytokines, chemokines, and growth factors. This multiplex technology can also be used to measure gene expression utilizing the Invitrogen QuantiGene Plex assays.
What are HLA antibodies?
HLA stands for Human Leukocyte Antigen. These antigens are proteins and they are found on most of the cells in our body. Each person has a unique set of HLA antigens. What is an antibody? Antibodies are also proteins and they are found in blood.
What can cause HLA antibodies?
What does high HLA mean?
People with certain HLA antigens are more likely to develop certain autoimmune diseases, such as type I diabetes, ankylosing spondylitis, rheumatoid arthritis, celiac disease, SLE (systemic lupus erythematosus), myasthenia gravis, inclusion body myositis, Sjögren syndrome, and narcolepsy.
Who bought Luminex?
DiaSorin
Italian diagnostics firm DiaSorin has concluded the acquisition of Luminex in an all-cash transaction for an equity value of nearly $1.8bn or $37 per share.
Why is HLA testing done?
The primary use for human leukocyte antigen (HLA) testing is to match organ and tissue transplant recipients with compatible donors. HLA testing also includes screening transplant recipients for the presence of antibodies that might target the donated tissue or organ as part of an immune response.
Is it bad to have HLA antibodies?
Are HLA antibodies harmful? HLA antibodies are not harmful to the person who made them. However, blood containing HLA antibodies may cause a rare but harmful reaction in some patients.
Which autoimmune disease is associated with HLA genes?
Strong association between the HLA region and autoimmune disease (AID) has been established for over fifty years. Association of components of the HLA class II encoded HLA-DRB1-DQA1-DQB1 haplotype has been detected with several AIDs, including rheumatoid arthritis, type 1 diabetes and Graves’ disease.
Why would I have HLA antibodies?
A: Anti-HLA antibodies are formed by the immune system when you are exposed to proteins that appear similar to tissue types. This most commonly occurs in the setting of previous transplantation, pregnancy, or blood transfusion. Occasionally the cause of anti-HLA antibody formation is not known.
When did DiaSorin acquire Luminex?
Saluggia, July 14, 2021 – DiaSorin S.p.A. (“DiaSorin”; FTSE MIB: DIA) announces it has completed the acquisition of Luminex Corporation (“Luminex”; NASDAQ: LMNX) for a price of USD 37.00 per share that corresponds to a total equity value of approximately USD 1.8 billion.
What is HLA in your blood?
HLA basics
Human leukocyte antigen (HLA) typing is used to match patients and donors for bone marrow or cord blood transplants. HLA are proteins — or markers — found on most cells in your body. Your immune system uses these markers to recognize which cells belong in your body and which do not.
What do my HLA results mean?
Specific HLA genes or antigens are identified during HLA testing to ensure solid organ or hematopoietic stem cell transplant compatibility. The typing result of the recipient is compared to those of the potential donor. Results indicate how many antigens match and how many antigen mismatches are present.